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Download fileSingle-Cell Copy Number Analysis of Prostate Cancer Cells Captured with Geometrically Enhanced Differential Immunocapture Microdevices
journal contribution
posted on 2014-11-18, 00:00 authored by Erica
D. Pratt, Asya Stepansky, James Hicks, Brian J. KirbyLimited access to tumor tissue makes
repeated sampling and real-time
tracking of cancer progression infeasible. Circulating tumor cells
(CTCs) provide the capacity for real-time genetic characterization
of a disseminating tumor cell population via a simple blood draw.
However, there is no straightforward method to analyze broadscale
genetic rearrangements in this heterogeneous cell population at the
single cell level. We present a one-step controllable chemical extraction
of whole nuclei from prostate cancer cells captured using geometrically
enhanced differential immunocapture (GEDI) microdevices. We have successfully
used copy number profile analysis to differentiate between two unique
cancer cell line populations of metastatic origin (LNCaP and VCaP)
and to analyze key mutations important in disease progression.
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Keywords
cell levelCTCCirculating tumor cellscancer cell line populationscell populationProstate Cancer Cellscopy number profile analysiscancer progression infeasiblemetastatic origintumor tissuetumor cell populationchemical extractionGEDIprostate cancer cellsImmunocapture MicrodevicesLimited accessdisease progressionGeometrically Enhanced