ac5b04749_si_001.pdf (1.57 MB)
Single-Cell Analysis Using Drop-on-Demand Inkjet Printing and Probe Electrospray Ionization Mass Spectrometry
journal contribution
posted on 2016-03-25, 00:00 authored by Fengming Chen, Luyao Lin, Jie Zhang, Ziyi He, Katsumi Uchiyama, Jin-Ming LinThis study describes a novel method
for single-cell analysis and
lipid profiling by combining drop-on-demand inkjet cell printing and
probe electrospray ionization mass spectrometry (PESI-MS). Through
inkjet sampling of a cell suspension, droplets with single cells were
generated, precisely dripped onto a tungsten-made electrospray ionization
needle, and immediately sprayed under a high-voltage electric field.
Lipid fingerprints of single cells were obtained by a mass spectrometry
(MS) detector. A homemade magnetic stirring device was applied to
the cell suspension reservoir, which controlled the homogeneous distribution
of cells in liquid and improved the single-cell-droplet percentage
by 43.8%. Eight types of single cells were screened in our platform
and further differentiated by principal component analysis based on
cellular surface phospholipids. Thus, this study successfully provides
a facile method for the direct MS profiling of single-cell lipids
by PESI-MS.