posted on 2019-01-08, 00:00authored byAndrew
S. Danis, Jesse B. Gordon, Karlie P. Potts, Lisa I. Stephens, Samuel C. Perry, Janine Mauzeroll
One of the long-standing
challenges to performing electrogenerated
chemiluminescence (ECL) research is the need for dedicated instrumentation
or highly customized cells to achieve reproducibility. This manuscript
describes an approach to designing ECL systems through the hyphenation
of existing laboratory instruments, which provide innate time correlation
of electrochemical and emission data. This design methodology lowers
the entry barrier required to obtaining reproducible ECL measurements
and provides flexibility in the scope of applications. Uniquely, the
simplicity of this system’s experimental interface, a spectrochemical
quartz cuvette, readily enables collaboration with finite element
modeling that simulates ECL occurring in the cuvette-based cell. This
combination of empirical and simulation data allowed for the investigation
of the intertwined kinetics behind the coreactant ECL mechanism of
tris(2,2′-bipyridine)ruthenium(II) (Ru(bpy)32+) and tripropylamine (TPA). The complexity of the system
measurable via the hyphenation methodology was further scaled though
the addition of tris[2-(4,6-difluorophenyl)pyridinato-C2, N] iridium(III) (Ir(dFppy)3) and the observation of
real time multiplexing.