Simultaneous Detection
of Ochratoxin A and Aflatoxins
in Industrial and Traditional Red and isot Pepper
Flakes along with Dietary Exposure Risk Assessment
posted on 2022-08-26, 15:12authored bySebahat Oztekin, Funda Karbancioglu-Guler
This study focused on the co-occurrence of aflatoxins
(AFs) and
ochratoxin A (OTA) in traditionally and industrially dried red pepper
flakes (DRPFs) and isot pepper flakes (IPFs). Following
the multitoxin immunoaffinity column (IAC) clean-up, high-performance
liquid chromatography coupled with fluorescence detection (HPLC-FLD)
was used to quantify AFs and OTA. The limit of detection (LOD) and
limit of quantification (LOQ) values were 0.11 and 0.18 μg kg–1 (AFB1), 0.04 and 0.08 μg kg–1 (AFB2), 0.13 and 0.18 μg kg–1 (AFG1), 0.04 and 0.11 μg kg–1 (AFG2), and 0.10 and 0.21 μg kg–1 (OTA), respectively. AFB1, AFB2, AFG1, and OTA were found to be positive in 93, 74, 17,
and 94% of all samples, respectively. The contamination levels in
positive samples ranged from 0.23 to 38.69, 0.04 to 2.14, 0.13 to
0.88, and 0.18 to 52.19 μg kg–1 for AFB1, AFB2, AFG1, and OTA, respectively,
while no AFG2 was found above the detection limit (0.04
μg kg–1). None of the industrial isot peppers exceeded the European Union limits, while the levels of
AFB1 (5 μg kg–1), total AFs (10
μg kg–1), and OTA (20 μg kg–1) of the traditional peppers were above the limit by 30% (16/54),
26% (14/54), and 4% (2/54), respectively. Co-occurrence of AFB1-AFB2-OTA was the most frequent, accounting for
54% (29/54) of all samples. At the upper bound (UB), estimated average
exposure to AFB1, total AFs, and OTA was determined to
be 0.175, 0.189, and 0.124 ng kg–1 bw day–1 in all samples, respectively. The margin of exposure (MoE) value
of AFB1 and total AFs was found to be 977 and 909, indicating
high health concerns compared to OTA (MoE >10,000). AFB1 and total AFs may result in 0.0058 and 0.0062 liver cancer cases/100,000
person/year at UB, respectively, and weekly OTA exposure was 0.868
ng kg–1 bw, well below the provisional tolerable
weekly intake, hence not of health concern. AFs exposure could endanger
health, whereas OTA posed no toxicological concerns through dried
red pepper consumption