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Simple Methods for Tracking Stem Cells with 64Cu-Labeled DOTA-hexadecyl-benzoate

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journal contribution
posted on 14.05.2015, 00:00 authored by Min Hwan Kim, Sang-Keun Woo, Kwang Il Kim, Tae Sup Lee, Chan Wha Kim, Joo Hyun Kang, Byung Il Kim, Sang Moo Lim, Kyo Chul Lee, Yong Jin Lee
The purpose of this study was to evaluate 64Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (64Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with 64Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.