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Silver Nanoparticle−Oligonucleotide Conjugates Based on DNA with Triple Cyclic Disulfide Moieties

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journal contribution
posted on 03.04.2020, 17:44 by Jae-Seung Lee, Abigail K. R. Lytton-Jean, Sarah J. Hurst, Chad A. Mirkin
We report a new strategy for preparing silver nanoparticle−oligonucleotide conjugates that are based upon DNA with cyclic disulfide-anchoring groups. These particles are extremely stable and can withstand NaCl concentrations up to 1.0 M. When silver nanoparticles functionalized with complementary sequences are combined, they assemble to form DNA-linked nanoparticle networks. This assembly process is reversible with heating and is associated with a red shifting of the particle surface plasmon resonance and a concomitant color change from yellow to pale red. Analogous to the oligonucleotide-functionalized gold nanoparticles, these particles also exhibit highly cooperative binding properties with extremely sharp melting transitions. This work is an important step toward using silver nanoparticle−oligonucleotide conjugates for a variety of purposes, including molecular diagnostic labels, synthons in programmable materials synthesis approaches, and functional components for nanoelectronic and plasmonic devices.