Silk Molecular Weight Influences the Kinetics of Enzymatically Cross-linked Silk Hydrogel Formation
journal contributionposted on 2018-11-13, 00:00 authored by A. Pasha Tabatabai, Benjamin P. Partlow, Nicole R. Raia, David L. Kaplan, Daniel L. Blair
We transform reconstituted silk solutions into robust hydrogels through covalent dityrosine cross-linking resulting from an enzymatic reaction. The bulk rheological properties and the covalent dityrosine bond formation of these gels are measured during polymerization. We compare the time-resolved bond formation to the mechanical properties, where we find that the gelation process is consistent with a model of percolation. The molecular weight of the protein determines whether a secondary mode of growth postpercolation exists, indicating that molecular weight changes affect the mechanisms by which these gels polymerize.
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silk Molecular Weight Influencesreconstituted silk solutionsbulk rheological propertiesweight changescovalent dityrosine cross-linkingEnzymatically Cross-linked Silk Hydrogel Formationproteinhydrogeltime-resolved bond formationmechanismpolymerizationgelation processgrowth postpercolationcovalent dityrosine bond formationpolymerizemodelmodeKinetic