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Si Nanowires Forest-Based On-Chip Biomolecular Filtering, Separation and Preconcentration Devices: Nanowires Do it All
journal contribution
posted on 2016-02-20, 12:00 authored by Vadim Krivitsky, Lo-Chang Hsiung, Amir Lichtenstein, Boris Brudnik, Raisa Kantaev, Roey Elnathan, Alexander Pevzner, Artium Khatchtourints, Fernando PatolskyThe development of efficient biomolecular separation
and purification
techniques is of critical importance in modern genomics, proteomics,
and biosensing areas, primarily due to the fact that most biosamples
are mixtures of high diversity and complexity. Most of existent techniques
lack the capability to rapidly and selectively separate and concentrate
specific target proteins from a complex biosample, and are difficult
to integrate with lab-on-a-chip sensing devices. Here, we demonstrate
the development of an on-chip all-SiNW filtering, selective separation,
desalting, and preconcentration platform for the direct analysis of
whole blood and other complex biosamples. The separation of required
protein analytes from raw biosamples is first performed using a antibody-modified
roughness-controlled SiNWs (silicon nanowires) forest of ultralarge
binding surface area, followed by the release of target proteins in
a controlled liquid media, and their subsequent detection by supersensitive
SiNW-based FETs arrays fabricated on the same chip platform. Importantly,
this is the first demonstration of an all-NWs device for the whole direct analysis of blood samples on a single chip,
able to selectively collect and separate specific low abundant proteins,
while easily removing unwanted blood components (proteins, cells)
and achieving desalting effects, without the requirement of time-consuming
centrifugation steps, the use of desalting or affinity columns. Futhermore,
we have demonstrated the use of our nanowire forest-based separation
device, integrated in a single platform with downstream SiNW-based
sensors arrays, for the real-time ultrasensitive detection of protein
biomarkers directly from blood samples. The whole ultrasensitive protein
label-free analysis process can be practically performed in less than
10 min.