Sensitive Oxidation of Sorbitol-Mediated Fe²⁺ by H₂O₂: A Reliable TD-NMR Method for Clinical Blood Glucose Detection

The clinical challenge of high-accuracy blood glucose detection schemes is to overcome the detection error caused by the background interferences in different individuals. H₂O₂ as the specific product of glucose oxidation can be involved in the Fe²⁺/Fe³⁺ conversion and detected by the time-domain nuclear magnetic resonance (TD-NMR) method sensitively. But, in clinical applications, the oxidation of Fe²⁺ is susceptible to the complex sample substrates. In this work, we sorted out two kinds of possible interference mechanisms of Fe²⁺ oxidation in the NMR blood glucose detection method and proposed a feasible scheme that uses sorbitol to weaken the adverse effects of interference. We found that sorbitol-mediated Fe²⁺ can greatly enhance the sensitivity of the <i>T</i>₂ value to H₂O₂. The chain reaction caused by sorbitol can significantly amplify the efficiency of Fe²⁺ oxidation at the same concentration of H₂O₂. Thereby, we can achieve the higher dilution multiple of serum samples to reduce the amount of interfering substances involved in the Fe²⁺/Fe³⁺ conversion. We justified the accuracy and availability of our method by successfully detecting and confirming the correlation between the <i>T</i>₂ decrease and glucose concentration of the serum samples collected from 16 subjects. The sorbitol–Fe²⁺ glucose detection method with high sensitivity can be further combined with miniature NMR analyzers to satisfy the calibration requirements of glucose monitoring in diabetic patients instead of frequent medical visits.