posted on 2021-12-02, 13:38authored byRafiq
M. Lubken, Max H. Bergkamp, Arthur M. de Jong, Menno W. J. Prins
Studies on the dynamics
of biological systems and biotechnological
processes require measurement techniques that can reveal time dependencies
of concentrations of specific biomolecules, preferably with small
time delays, short time intervals between subsequent measurements,
and the possibility to record over long time spans. For low-concentration
biomolecules, these requirements are very challenging since low-concentration
assays are typically slow and require new reagents in every assay.
Here, we present a sensing methodology that enables rapid monitoring
of picomolar and sub-picomolar concentrations in a reversible affinity-based
assay, studied using simulations. We demonstrate that low-concentration
biomolecules can be monitored with small time delays, short time intervals,
and in principle over an endless time span.