am7b09071_si_001.pdf (5.51 MB)
Self-Reporting Photoluminescent Porous Silicon Microparticles for Drug Delivery
journal contribution
posted on 2017-12-26, 00:00 authored by Joanna Wang, Tushar Kumeria, Maria Teresa Bezem, Jian Wang, Michael J. SailorA porous
Si (pSi) microparticle-based delivery system is investigated, and
the intrinsic luminescence from the particles is employed as a probe
to monitor the release of a model protein payload, bovine serum albumin
(BSA). The microparticles consist of a core Si skeleton surrounded
by a SiO2 shell. Two types of pSi are tested, one with
smaller (10 nm) pores and the other with larger (20 nm) pores. The
larger pore material yields a higher mass loading of BSA (3 vs 20%).
Two different methods are used to load BSA into these nanostructures:
the first involves loading by electrostatic physisorption, and the
second involves trapping of BSA in the pSi matrix by local precipitation
of magnesium silicate. Protein release from the former system is characterized
by a burst release, whereas in the latter system, release is controlled
by dissolution of the pSi/magnesium silicate matrix. The protein release
characteristics are studied under accelerated (0.1 M aqueous KOH,
21 °C) and physiologically relevant (phosphate-buffered saline,
pH 7.4, 37 °C) conditions, and the near-infrared photoluminescence
signal from the pSi skeleton is monitored as a function of time and
correlated with protein release and silicon dissolution. The thickness
of the Si core and the SiO2 shell are systematically varied,
and it is found that the luminescence signature can be tuned to provide
a signal that either scales with protein elution or that changes rapidly
near the end of useful life of the delivery system. Although payload
release and particle dissolution are not driven by the same mechanism,
the correlations between luminescence and payload elution for the
various formulations can be used to define design rules for this self-reporting
delivery system.
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Keywords
protein releaseSelf-Reporting Photoluminescent Porous Silicon Microparticlessilicon dissolutionmass loadingdesign rulespayload elutionmicroparticle-based delivery systemDrug DeliverySi coreprotein release characteristicsProtein releasepayload releasecore Si skeletondelivery systemmagnesium silicatepSi skeletonparticle dissolutionpore material yieldsnear-infrared photoluminescence signalKOHphosphate-buffered salineload BSAluminescence signatureSiO 2 shellburst releaseself-reporting delivery systemmodel protein payloadprotein elutionpSi matrixserum albuminlatter system
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