nn7b03739_si_001.pdf (2.03 MB)
Self-Assembly of Mesoscale Artificial Clathrin Mimics
journal contribution
posted on 2017-09-18, 00:00 authored by Yifan Kong, Mina-Elraheb S. Hanna, Denys Zhuo, Katherine G. Chang, Tara Bozorg-Grayeli, Nicholas A. MeloshFluidic control and
sampling in complex environments is an important
process in biotechnology, materials synthesis, and microfluidics.
An elegant solution to this problem has evolved in nature through
cellular endocytosis, where the dynamic recruitment, self-assembly,
and spherical budding of clathrin proteins allows cells to sample
their external environment. Yet despite the importance and utility
of endocytosis, artificial systems which can replicate this dynamic
behavior have not been developed. Guided by clathrin’s unusual
structure, we created simplified metallic microparticles that capture
the three-legged shape, particle curvature, and interfacial attachment
characteristics of clathrin. These artificial clathrin mimics successfully
recreate biomimetic analogues of clathrin’s recruitment, assembly,
and budding, ultimately forming extended networks at fluid interfaces
and invaginating immiscible phases into spheres under external fields.
Particle curvature was discovered to be a critical structural motif,
greatly limiting irreversible aggregation and inducing the legs’
selective tip-to-tip attraction. This architecture provides a template
for a class of active self-assembly units to drive structural and
dimensional transformations of liquid–liquid interfaces and
microscale fluidic sampling.
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self-assembly unitsrecruitmentendocytosimaterials synthesisbiomimetic analoguesclathrin proteinsparticle curvaturetip-to-tip attractionclathrin mimicsattachment characteristicsClathrin Mimics Fluidic controlfluid interfacesthree-legged shapemicroscale fluidic samplinginvaginating immiscible phasesParticle curvature
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