American Chemical Society
am8b07889_si_001.pdf (1.17 MB)

Self-Assembled Double-Bundle DNA Tetrahedron for Efficient Antisense Delivery

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journal contribution
posted on 2018-07-02, 00:00 authored by Juanjuan Yang, Qiao Jiang, Lin He, Pengfei Zhan, Qing Liu, Shaoli Liu, Meifang Fu, Jianbing Liu, Can Li, Baoquan Ding
DNA nanostructures are promising biomaterials capable of arranging multiple functional components with nanometer precision. Here, a double-bundle DNA tetrahedron is rationally designed to integrate with antisense oligonucleotides silencing proto-oncogene c-raf and nuclear targeting peptides. The functionalized DNA tetrahedron can be internalized by A549 cells and assists the delivery of antisense oligonucleotides toward the nucleus to increase the chance to downregulate target mRNA in nucleus and cytoplasm. Antisense strands released from the tetrahedron in response to the intracellular reducing environment can inhibit cell proliferation at a low concentration without transfection reagent. Finally, efficient knockdown of c-raf gene is observed, which verified our design. This designer DNA-based nanocarrier system will open a new avenue for efficient delivery of nucleic acid drugs.