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Selective Sensing of Peroxynitrite by Hf-Based UiO-66-B(OH)2 Metal–Organic Framework: Applicability to Cell Imaging
journal contribution
posted on 2018-08-09, 18:22 authored by Mostakim SK, Soutick Nandi, Rakesh Kumar Singh, Vishal Trivedi, Shyam BiswasThe first boronic acid functionalized
Hf-based UiO-66 (UiO = University
of Oslo) metal–organic framework (MOF) having the ability to
detect both extracellular and intracellular peroxynitrite is presented.
The Hf-UiO-66-B(OH)2 material (1) was synthesized
under solvothermal conditions from a mixture of HfCl4 and
2-borono-1,4-benzenedicarboxylic acid [H2BDC–B(OH)2] ligand in DMF in the presence of formic acid (modulator)
at 130 °C for 48 h. The desolvated material (1′) was utilized as a fluorescent turn-on probe for the rapid sensing
of extracellular peroxynitrite (ONOO–) under conditions
mimicking those of biological medium (10 mM HEPES buffer, pH 7.4).
Selective sensing of ONOO– over other ROS/RNS was
also achieved by 1′. The oxidative cleavage of
attached boronic acid groups forming corresponding hydroxy-functionalized
ligands can be accounted for the fluorescent increment phenomenon
in the presence of ONOO–. The probe showed extraordinary
sensitivity (detection limit = 9.0 nM) toward ONOO– in 10 mM HEPES buffer at pH 7.4. Probe-loaded cells did not exhibit
cytotoxicity and morphological deformities. It is remarkable that
the probe inside the cells responded toward the peroxynitrite solution
to give an intense blue fluorescent signal. The fluorescence microscopy
study with J774A.1 macrophage cells unambiguously demonstrated that
probe 1′ is suitable to image peroxynitrite in
living cells.
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extracellular peroxynitrite48 hJ 774A macrophage cellsdetection limitHf-UiO -66-B 2 materialperoxynitrite solution10 mM HEPES buffersolvothermal conditionsdesolvated materialexhibit cytotoxicityoxidative cleavageROSBDCCell Imagingturn-on probefluorescence microscopy studyMOFpresenceDMFONOOintracellular peroxynitriteboronic acid groupsHfCl 4increment phenomenonformic acidhydroxy-functionalized ligands9.0 nMimage peroxynitritepH 7.4. Probe-loaded cellsboronic acid functionalized Hf-based UiO -66
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