Selective Naked-Eye Detection of Hg2+ through an Efficient Turn-On Photoinduced Electron Transfer Fluorescent Probe and Its Real Applications
journal contributionposted on 02.09.2014, 00:00 by Priyanka Srivastava, Syed S. Razi, Rashid Ali, Ramesh C. Gupta, Suresh S. Yadav, Gopeshwar Narayan, Arvind Misra
A simple molecular fluorescent probe 5 has been designed and synthesized by appending anthracene and benzhydryl moieties through a piperazine bridge. The probe upon interaction with different metal ions showed high selectivity and sensitivity (2 ppb) for Hg2+ through fluorescence “turn-on” response in HEPES buffer. The significant fluorescence enhancement (∼10-fold) is attributable to PET arrest due to complexation with nitrogen atoms of the piperazine unit and Hg2+ in 1:2 stoichiometry, in which a naked-eye sensitive fluorescent blue color of solution changed to a blue-green (switched-on). As a proof of concept, promising prospects for application in environmental and biological sciences 5 have been utilized to detect Hg2+ sensitively in real samples, on cellulose paper strips, in protein medium (like BSA), and intracellularly in HeLa cells. Moreover, the optical behavior of 5 upon providing different chemical inputs has been utilized to construct individual logic gates and a reusable combinational logic circuit. The combinational circuit (switch ON mode; OR logic gate) is easily resettable to the original position (switch OFF mode; INHIBIT logic gate) by applying reset chemical inputs (OH– and PO43–) with great reproducibility.
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HEPES bufferlogic gateReal ApplicationsApiperazine unitswitch OFF modePET arrestlogic gatesOHreset chemical inputsnitrogen atomspiperazine bridgesciences 5appending anthraceneHgcombinational logic circuitbenzhydryl moietiesHeLa cellscellulose paper stripschemical inputsPOprobe 5BSAfluorescenceprotein mediummetal ionscombinational circuitINHIBIT logic gate