ac0505223_si_001.pdf (109.61 kB)
Download fileSelective Immobilization of Fusion Proteins on Poly(hydroxyalkanoate) Microbeads
journal contribution
posted on 2005-09-01, 00:00 authored by Seok Jae Lee, Jong Pil Park, Tae Jung Park, Sang Yup Lee, Seongnam Lee, Jung Ki ParkA novel fusion protein system employing the substrate-binding domain (SBD) of poly(hydroxyalkanoate) (PHA)
depolymerase was developed for the specific immobilization of proteins on PHA microbeads, and was consequently used for immunoassays. The enhanced green
fluorescent protein, red fluorescent protein, and severe
acute respiratory syndrome coronavirus envelope protein
were used as model proteins, and were selectively and
functionally immobilized to the PHA microbeads by fusing
them to the SBD. Using this PHA microbead system
combined with SBD fusion technology, immunoassays
could be successfully carried out.