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Selective Enrichment of Cysteine-Containing Peptides Using SPDP-Functionalized Superparamagnetic Fe3O4@SiO2 Nanoparticles: Application to Comprehensive Proteomic Profiling

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journal contribution
posted on 01.08.2008, 00:00 by Arudra Palani, Je-Sun Lee, Jiyoung Huh, Minsik Kim, Yeon-Ji Lee, Jeong Ho Chang, Kwangyeol Lee, Sang-Won Lee
Superparamagnetic Fe3O4@SiO2 core−shell nanoparticles (ca. 30 nm diameter), which are surface-modified with a thiol-specific functional group, exhibit superb capturing efficiency toward cysteinyl peptides without contamination from nonspecifically interacting peptides, as clearly evidenced through LC/MS/MS analysis.