ja3122312_si_001.pdf (18.77 MB)
Download fileSelective Cell Death by Photochemically Induced pH Imbalance in Cancer Cells
journal contribution
posted on 2016-02-19, 23:05 authored by Xiling Yue, Ciceron
O. Yanez, Sheng Yao, Kevin D. BelfieldSinglet oxygen sensitized photodynamic therapy (PDT)
relies on
the concentration of oxygen in the tissue to be treated. Most cancer
lesions, however, have poor vasculature and, as a result, are hypoxic,
significantly hindering PDT efficacies. An oxygen-independent PDT
method may circumvent this limitation. To address this, we prepared
sulfonium salts that produced a pH drop within HCT 116 cells via the
generation of a photoacid within the cytosol. This process was driven
by one- or two-photon absorption (1PA or 2PA) of the endocytosed photoacid
generators (PAGs). One of these PAGs, which had a significantly lower
dark cytotoxicity and was more efficient in generating a photoacid,
effectively induced necrotic cell death in the HCT 116 cells. The
data suggest that PAGs may be an attractive alternative PDT modality
to selectively induce cell death in oxygen-deprived tissue such as
tumors.