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Selective Assembly and Alignment of Actin Filaments with Desired Polarity on Solid Substrates

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posted on 2006-10-10, 00:00 authored by Ling Huang, Pradeep Manandhar, Kyung-Eun Byun, P. Bryant Chase, Seunghun Hong
We report a new strategy to selectively assemble and align filamentous actin (F-actin) onto desired locations on a solid substrate with a specific structural polarity. In this strategy, biotinylated gelsolin caps the structural minus end of F-actin so that the F-actin binds onto a streptavidin pattern with a specific structural polarity. We also demonstrate that an electric field can be utilized to align bound F-actin along a desired direction. This can be one of the major technical breakthroughs toward the assembly of nanomechanical systems based on myosin biomotors.

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