posted on 2019-03-14, 00:00authored byAdrian
J. Jervis, Pablo Carbonell, Sandra Taylor, Rehana Sung, Mark S. Dunstan, Christopher J. Robinson, Rainer Breitling, Eriko Takano, Nigel S. Scrutton
The rapid prototyping and optimization
of plasmid-based recombinant
gene expression is one of the key steps in the development of bioengineered
bacterial systems. Often, multiple genes or gene modules need to be
coexpressed, and for this purpose compatible, inducible plasmid systems
have been developed. However, inducible expression systems are not
favored in industrial processes, due to their prohibitive cost, and
consequently the conversion to constitutive expression systems is
often desired. Here we present a set of constitutive-expression plasmids
for this purpose, which were benchmarked using fluorescent reporter
genes. To further facilitate the conversion between inducible and
constitutive expression systems, we developed SelProm, a design tool
that serves as a parts repository of plasmid expression strength and
predicts portability rules between constitutive and inducible plasmids
through model comparison and machine learning. The SelProm tool is
freely available at http://selprom.synbiochem.co.uk.