Sea Anemone-like
Nanomachine Based on DNA Strand Displacement
Composed of Three Boolean Logic Gates: Diversified Input for Intracellular
Multitarget Detection
posted on 2024-02-27, 18:54authored byYuting Li, Peng Zhou, Zhenxin Wang, Yiping Ren, Xu Zhu, Jin Wang, Hanrong Yan, Lei Hua, Fenglei Gao
Efficient
and accurate acquisition of cellular biomolecular
information
is crucial for exploring cell fate, achieving early diagnosis, and
the effective treatment of various diseases. However, current DNA
biosensors are mostly limited to single-target detection, with few
complex logic circuits for comprehensive analysis of three or more
targets. Herein, we designed a sea anemone-like DNA nanomachine based
on DNA strand displacement composed of three logic gates (YES-AND-YES)
and delivered into the cells using gold nano bipyramid carriers. The
AND gate activation depends on the trigger chain released by upstream
DNA strand displacement reactions, while the output signal relies
on the downstream DNAzyme structure. Under the influence of diverse
inputs (including enzymes, miRNA, and metal ions), the interconnected
logic gates simultaneously perform logical analysis on multiple targets,
generating a unique output signal in the YES/NO format. This sensor
can successfully distinguish healthy cells from tumor cells and can
be further used for the diagnosis of different tumor cells, providing
a promising platform for accurate cell-type identification.