posted on 2023-12-20, 06:29authored byHuan Wen, Sining Zhang, Yuan Liu, Zhehui Hu, Congyi Zhu, Jiwu Zeng, Zhiqing Song, Jiajing Chen, Juan Xu
Volatile
terpenoids accumulate in citrus and play important roles
in plant defense against various stressors. However, the broad-spectrum
response of terpenoid biosynthesis to ubiquitous stressors in citrus
has not been comparatively investigated. In this study, volatile terpenoids
were profiled under six stressors: high temperature, citrus miner,
citrus red mite, citrus canker, Alternaria brown spot, and huanglongbing
(HLB). Significant content changes in 15 terpenoids, including β-ocimene,
were observed in more than four of the six stressors, implying their
possibly universal stress-response effects. Notably, the emission
of terpenoids, including β-caryophyllene, β-ocimene, and
nerolidol glucoside, was significantly increased by HLB in HLB-tolerant
“Shatian” pomelo leaves. The upregulation of CgTPS1 and CgTPS2 and their characterization in vivo identified them as mono- or sesquiterpenoid biosynthetic
genes. This study provides a foundation for determining stress resistance
mechanisms in citrus and biopesticide designations for future industrial
applications.