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Ru(II) Glycodendrimers as Probes to Study Lectin−Carbohydrate Interactions and Electrochemically Measure Monosaccharide and Oligosaccharide Concentrations

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journal contribution
posted on 02.02.2010, 00:00 by Raghavendra Kikkeri, Faustin Kamena, Tarkeshwar Gupta, Laila H. Hossain, Siwarutt Boonyarattanakalin, Ganna Gorodyska, Eva Beurer, Géraldine Coullerez, Marcus Textor, Peter H. Seeberger
We describe a novel platform on which to study carbohydrate−protein interactions based on ruthenium(II) glycodendrimers as optical and electrochemical probes. Using the prototypical concanavalin A (ConA)−mannose lectin−carbohydrate interaction as an example, oligosaccharide concentrations were electrochemically monitored. The displacement of the Ru(II) complex from lectin-functionalized gold surfaces was repeatedly regenerated. This new platform presents a method to monitor many different complex sugars in parallel.