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Robust Heterochiral Strand Displacement Using Leakless Translators
journal contribution
posted on 2020-07-01, 20:34 authored by Tracy
L. Mallette, Milan N. Stojanovic, Darko Stefanovic, Matthew R. LakinMolecular computing offers a powerful
framework for in
situ biosensing and signal processing at the nanoscale. However,
for in vivo applications, the use of conventional
DNA components can lead to false positive signals being generated
due to degradation of circuit components by nuclease enzymes. Here,
we use hybrid chiral molecules, consisting of both l- and d-nucleic acid domains, to implement leakless signal translators
that enable d-nucleic acid signals to be detected by hybridization
and then translated into a robust l-DNA signal for further
analysis. We show that our system is robust to false positive signals
even if the d-DNA components are degraded by nucleases, thanks
to circuit-level robustness. This work thus broadens the scope and
applicability of DNA-based molecular computers for practical, in vivo applications.
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