Reconstitution of Functionalized Transmembrane Domains of Receptor Proteins into Biomimetic Membranes
journal contributionposted on 25.08.2015, 00:00 by Daniel R. Scott, Vitalii Silin, Hirsh Nanda
For integral membrane proteins, an assessment of their structures and interactions within a biomimetic lipid bilayer environment is critical for evaluating their cellular function. Hydrophobic sequences prevalent within transmembrane domains, however, make these proteins susceptible to aggregation and, thus, create difficulties in examining their structural and functional properties via canonical techniques. Working exclusively with single-pass transmembrane (TM) segments of bitopic membrane proteins, in the form of soluble peptides, bypasses many of the pitfalls of full-length protein preparations while allowing for the opportunity to examine the properties of TM domains within biomimetic membrane environments. In this study, peptides mimicking the TM domains of the epidermal growth factor receptor (EGFR) and CD4 co-receptor, both cell-signaling surface receptors, have been reconstituted into 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid bilayers. The formation of their native α-helical structures within vesicle membranes was observed from circular dichroism, and full partition of the peptides into the membrane was demonstrated by tryptophan fluorescence and neutron reflectivity (NR). Using an engineered planar lipid bilayer system ideal for surface characterization methods, such as surface plasmon resonance (SPR) and NR, the TM peptides, functionalized with a N-terminal biotin tag, proved capable of “activating” a membrane surface, as evidenced by the capture of streptavidin. On the basis of these initial assessments, we anticipate these membrane-bound peptides will provide a versatile platform for understanding the intricate roles of receptor TM domains in cell signaling.
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POPCTM domainsbiomimetic lipid bilayer environmentlipid bilayer systemSPRpeptideepidermal growth factor receptorsurface characterization methodsbitopic membrane proteinsbiomimetic membrane environmentsNRsurface plasmon resonanceEGFRreceptor TM domainsFunctionalized Transmembrane DomainsBiomimetic MembranesFor integral membrane proteins