Realization of a Model-Free Pathway for Quantum Dot–Protein Interaction Beyond Classical Protein Corona or Protein Complex

Although in recent times nanoparticles (NPs) are being used in various biological applications, their mechanism of binding interactions still remains hazy. Usually, the binding mechanism is perceived to be mediated through either the protein corona (PC) or protein complex (PCx). Herein, we report that the nanoparticle (NP)–protein interaction can also proceed via a different pathway without forming the commonly observed PC or PCx. In the present study, the NP–protein interaction between less-toxic zinc–silver–indium-sulfide (ZAIS) quantum dots (QDs) and bovine serum albumin (BSA) was investigated by employing spectroscopic and microscopic techniques. Although the analyses of data obtained from fluorescence and thermodynamic studies do indicate the binding between QDs and BSA, they do not provide clear experimental evidence in favor of PC or PCx. Quite interestingly, high-resolution transmission electron microscopy (HRTEM) studies have shown the formation of a new type of species where BSA protein molecules are adsorbed onto some portion of a QD surface rather than the entire surface. To the best of our knowledge, we believe that this is the first direct experimental evidence in favor of a model-free pathway for NP–protein interaction events. Thus, the outcome of the present study, through experimental evidence, clearly suggests that NP–protein interaction can proceed by following a pathway that is different from classical PC and PCx.