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Real-Time Analysis of Porphyrin J‑Aggregation on a Plant-Esterase-Functionalized Surface Using Quartz Crystal Microbalance with Dissipation Monitoring

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journal contribution
posted on 26.08.2014, 00:00 by Limin Yang, Lei Jiang, Weijing Yao, Junling Liu, Juan Han
The J-aggregation of meso-tetra (4-sulfonatophenyl) porphine (TPPS4) on a plant-esterase-functionalized surface in a 1:1 v/v mixture of 0.05 M HCl/ethanol (pH ∼1.38) was analyzed in real time using a quartz crystal microbalance with dissipation monitoring (QCM-D). Simultaneous changes in frequency (Δf) and energy dissipation (ΔD) correlated well with mass and structural changes during the sequential phases of slow nucleation, rapid aggregation, and equilibration in J-aggregation. The time-dependent mass adsorption could be quantitatively analyzed with a model, which integrated two simple equations obtained when the surface concentration of TPPS4TPPS4) was below and above the critical aggregation surface concentration (CASC). This study provides a new view for the protein-induced J-aggregation process, which may be helpful for understanding the interactions of self-assembled nanostructures with biomolecules.