jz0c02029_si_001.pdf (1.25 MB)
Real-Time Microscale Temperature Imaging by Stimulated Raman Scattering
journal contribution
posted on 2020-08-17, 16:36 authored by Benjamin Figueroa, Ruoqian Hu, Samuel G. Rayner, Ying Zheng, Dan FuMicroscale thermometry of aqueous
solutions is essential to understand
the dynamics of local heat generation and dissipation in chemical
and biological systems. A wide variety of fluorescent probes have
been developed to map temperature changes with submicrometer resolution,
but they often suffer from the uncertainty associated with microenvironment-dependent
fluorescent properties. In this work, we develop a label-free ratiometric
stimulated Raman scattering (SRS) microscopy technique to quantify
microscale temperature by monitoring the O–H Raman stretching
modes of water. By tracking the ratio changes of the hydrogen-bonding
O–H band and the isosbestic band, we can directly quantify
the temperature of water-based environments in real time without exogenous
contrast agents. We demonstrate real-time measurement of localized
intracellular and extracellular temperature changes due to laser absorption.
This high-speed nonlinear optical imaging technique has the potential
for in situ microscale imaging of thermogenesis in
both chemical and biological systems.
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isosbestic bandlaser absorptionextracellular temperature changeslabel-free ratiometricheat generationratio changeschemicalexogenous contrast agentsStimulated Raman Scattering Microsc...water-based environmentsSRSReal-Time Microscale Temperature Im...microscale imagingmicroscale temperaturemicroscopy techniquesubmicrometer resolutionimaging techniquemap temperature changes
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