Rational Modulation of the Luminescence of Upconversion
Nanomaterials with Phycocyanin for the Sensing and Imaging of Myeloperoxidase
during an Inflammatory Process
posted on 2020-03-13, 15:17authored byYi You, Shasha Cheng, Li Zhang, Yingxin Zhu, Cuiling Zhang, Yuezhong Xian
Myeloperoxidase (MPO)
is a heme peroxidases protein associated
with many inflammation-related diseases. Although many fluorescent
probes have been constructed for the assessment of MPO activity, it
still remains a challege to develop a nanoprobe for highly sensitive
biosensing and high-resolution bioimaging in biological system. In
this work, we developed a novel luminescent nanoprobe based on upconversion
nanoparticles (UCNPs) conjugated with phycocyanin (PC), which could
detect the fluctuation of MPO. By grafting PC onto the surface of
UCNPs through amidation reaction, the luminescence of UCNPs is quenched
by PC via energy transfer. Due to the specific recognition by PC,
the nanoprobe can be used for sensitive evaluation the bioactivity
of MPO. The nanoprobe based on PC-UCNPs has been successfully applied
for the bioimaging of MPO in living cells and an inflammatory process
by taking an acute liver injury mouse as a model.