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Rapid and Quantitative Sizing of Nanoparticles Using Three-Dimensional Single-Particle Tracking

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journal contribution
posted on 11.01.2007, 00:00 by C. Shan Xu, Hu Cang, Daniel Montiel, Haw Yang
We report the first application of three-dimensional (3D) single-particle tracking (SPT) to hydrodynamic size characterization of gold nanoparticles in water. Nanoparticles undergoing Brownian motion were dynamically locked at the focal point of a microscope objective, one at a time, by rapid counteractive movements of the sample container. The hydrodynamic radius was derived from the recorded trajectory of each individual nanoparticle. The directly measured size and size distribution using 3D-SPT were in agreement with those obtained using the conventional dynamic light scattering (DLS) and using transmission electron microscopy (TEM), respectively.