posted on 2024-01-12, 15:03authored bySiqi Wei, Qing Tang, Xiumei Hu, Wei Ouyang, Huaze Shao, Jincheng Li, Hong Yan, Yue Chen, Lihong Liu
Rapid
prescribing of the right antibiotic is the key to treat infectious
diseases and decelerate the challenge of bacterial antibiotic resistance.
Herein, by targeting the 16S rRNA of bacteria, we developed a cation
dye-triggered electrokinetic gold nanoparticle (AuNP) agglutination
(CD-TEAA) method, which is rapid, visual, ultrasensitive, culture-independent,
and low in cost. The limit of detection (LOD) is as low as 1 CFU mL–1 Escherichia coli.
The infection identifications of aseptic fluid samples (n = 11) and urine samples with a clinically suspected urinary tract
infection (UTI, n = 78) were accomplished within
50 and 30 min for each sample, respectively. The antimicrobial susceptibility
testing (AST) of UTI urine samples was achieved within 2.5 h. In ROC
analysis of urine, the sensitivity and specificity were 100 and 96%
for infection identification, and 100 and 98% for AST, respectively.
Moreover, the overall cost of materials for each test is about US$0.69.
Therefore, the CD-TEAA method is a superior approach to existing,
time-consuming, and expensive methods, especially in less developed
areas.