Version 2 2022-12-28, 20:43Version 2 2022-12-28, 20:43
Version 1 2022-12-27, 14:34Version 1 2022-12-27, 14:34
journal contribution
posted on 2022-12-28, 20:43authored byFenghai Zhang, Xiaotong Wang, Ting Zhang, Zhe Zhang, Xin Gao, Yang Li
The receptor-binding domain of the SARS-CoV-2 spike mediates
the
key to binding the virus to the host receptor, but capturing the molecular
signal of this spike RBD remains a formidable challenge. Here, we
report a new surface-enhanced Raman spectroscopy (SERS) approach,
which used gold nanoparticles prepared by low-speed constant-temperature
centrifugation by bromine and calcium ions in two cleaning steps as
the enhanced substrate to rapidly and accurately detect spike RBD
large protein molecules in body fluids. The detection signal was extremely
stable, and the orientation of the spike RBD on the enhanced substrate
surface was also determined. This approach was specific in distinguishing
different SARS-CoV-2 variants of spike RBD, including Delta, Beta,
Gamma, and Omicron. Additionally, the enhanced substrate can identify
biologically active or inactive spike RBD. This two-step cleaning
enhanced substrate opens up opportunities not only for early diagnostics
of SARS-CoV-2 virus but also for developing targeted drugs against
viruses.