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Radioiodinated Nitroxide Derivative for the Detection of Lipid Radicals

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journal contribution
posted on 06.12.2019, 19:33 by Toshihide Yamasaki, Risa Azuma, Kohei Sano, Masayuki Munekane, Yuta Matsuoka, Ken-ichi Yamada, Takahiro Mukai
Thus far, no accurate measurement technology has been developed to detect lipid alkyl radicals (lipid radicals), which cause lipid peroxidation. Therefore, we aimed to develop a nuclear medical imaging probe that can be taken up in the lipophilic site in cells such as biological membranes, by reacting specifically with the lipid radicals generated there. We designed and synthesized 4-(4-[125I]­iodobenzamido)-2,2,6,6-tetramethylpiperidine-1-oxyl, which shows high reactivity to lipid radicals with a high radiochemical yield and purity. Intracellular retention was found to increase significantly when lipid radicals were produced.