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RNase I Modulates Escherichia coli Motility, Metabolism, and Resistance
journal contribution
posted on 2020-07-02, 18:35 authored by Yashasvika Duggal, Benjamin M. Fontaine, Deanna M. Dailey, Gang Ning, Emily E. WeinertBacteria are constantly
adapting to their environment by sensing
extracellular factors that trigger production of intracellular signaling
molecules, known as second messengers. Recently, 2′,3′-cyclic
nucleotide monophosphates (2′,3′-cNMPs) were identified
in Escherichia coli and have emerged as possible
novel signaling molecules. 2′,3′-cNMPs are produced
through endonucleolytic cleavage of short RNAs by the T2 endoribonuclease,
RNase I; however, the physiological roles of RNase I remain unclear.
Our transcriptomic analysis suggests that RNase I is involved in modulating
numerous cellular processes, including nucleotide metabolism, motility,
acid sensitivity, metal homeostasis, and outer membrane morphology.
Through a combination of deletion strain and inhibitor studies, we
demonstrate that RNase I plays a previously unknown role in E. coli stress resistance by affecting pathways that are
part of the defense mechanisms employed by bacteria when introduced
to external threats, including antibiotics. Thus, this work provides
insight into the emerging roles of RNase I in bacterial signaling
and physiology and highlights the potential of RNase I as a target
for antibacterial adjuvants.
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extracellular factorsmembrane morphologyEscherichia coliResistance Bacteriadefense mechanismsdeletion strainModulates Escherichia coli Motilityrolenucleotide metabolismendonucleolytic cleavageT 2 endoribonucleasemetal homeostasisRNaseRNAacid sensitivitytranscriptomic analysiscoli stress resistanceinhibitor studies
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