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Quantitative Proteomic Analysis Identified Paraoxonase 1 as a Novel Serum Biomarker for Microvascular Invasion in Hepatocellular Carcinoma
journal contribution
posted on 2013-04-05, 00:00 authored by Cheng Huang, Yuwei Wang, Shengdong Liu, Guangyu Ding, Weiren Liu, Jian Zhou, Ming Kuang, Yuan Ji, Tadashi Kondo, Jia FanThis
study aimed to identify serum biomarkers for microvascular invasion
(MVI) in hepatocellular carcinoma (HCC). MVI is a histological sign
of micrometastasis in the liver and is considered as one of the most
powerful prognostic factors in HCC. The serum of HCC patients with
different vascular invasion statuses was examined by iTRAQ-based proteomic
profiling. The expression levels of 24 proteins were associated with
the extent of vascular invasion in the pooled samples of 45 HCC cases.
Western blot analyses in 90 HCC cases confirmed the correlation of
the expression level of paraoxonase 1 (PON1) with the extent of vascular
invasion. ELISA assays demonstrated the diagnostic utility of the
PON1 level, with the area under curve values of 0.847 and 0.889 for
the MVI and gross vascular invasion, respectively, relative to the
patients without vascular invasion, in a cohort of 387 additional
HCC cases. Immunohistochemistry revealed that PON1 expression in tumor
cells was inversely correlated with the extent of vascular invasion
in 200 additional HCC cases. In conclusion, using a proteomic approach,
we found that serum PON1 was a novel diagnostic biomarker for MVI.
The prognostic values of serum PON1 and its possible therapeutic applications
are worth further investigation.