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Quantitative Phase Analysis of Complex Fats during Crystallization
journal contribution
posted on 2020-06-30, 17:05 authored by Naomi Arita MerinoNaomi Arita Merino, Hein van Valenberg, Elliot Paul Gilbert, Elke ScholtenThe
quantitative study of polymorphism in fats is complicated due
to their complex composition and consequent intricate crystallization
behavior. We aimed to illustrate how quantitative phase analysis can
be performed in crystallizing complex fats. The advantages of our
proposed method are that detailed structural information or internal
standards are not needed and that it can be applied even to wide-angle
X-ray diffraction patterns with a high noncrystalline (liquid fat)
contribution and overlapping peaks. Diffraction patterns of palm oil
and anhydrous milk fat during crystallization were decomposed by fitting
a model based on Pearson type VII functions. From the decomposed patterns,
the contribution of each phase was quantified as a function of time.
Each contribution to the pattern was then converted to its mass fraction
using response factors derived from the corresponding solid fat content,
which was measured using low-resolution nuclear magnetic resonance
(NMR). This approach allowed us to quantify the evolution of liquid
and polymorphic phases during the early crystallization of complex
fats and, for the first time, to quantify coexisting α, β′,
and β phases. This method is flexible enough for fats with diverse
triacylglycerol profiles yet detailed enough to quantify phase transitions
in a system as complex as milk fat.