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Download fileQuantitative Determination of Bulk Molecular Concentrations of β‑Agonists in Pork Tissue Samples by Direct Internal Extractive Electrospray Ionization-Mass Spectrometry
journal contribution
posted on 2017-10-09, 00:00 authored by Jiaquan Xu, Shengrui Xu, Yipo Xiao, Konstantin Chingin, Haiyan Lu, Runhan Yan, Huanwen ChenRapid
quantitative determination of bulk molecular concentration
in solid samples without sample pretreatment is demonstrated using
the internal extractive electrospray ionization-mass spectrometry
(iEESI-MS) analysis of six β-agonists, including salbutamol
(Sal), clenbuterol (Cle), ractopamine (Rac), terbutaline (Ter), tulobuterol
(Tul), brombuterol (Bro), in pork tissue samples. Single sample analysis
only required 1 min. The linear range of detection was about 0.01–1000
μg/kg (R2 > 0.9994). The limit-of-detection
(LOD) varied from 0.002 μg/kg for Sal to 0.006 μg/kg for
Tul. Relative standard deviation (RSD) of quantitation was in the
range 6.5–11.3%. The analytical results were validated by gas
chromatography–mass spectrometry (GC–MS) and high-performance
liquid chromatography–mass spectrometry (LC–MS), showing
the accuracy rates of 92–105%. The current study extends the
power of ambient MS as a method for the quantification of molecules
at the surface of solid samples (e.g., in μg/cm2 units)
toward the quantification of molecules in bulk sample volume (i.e.,
in μg/kg units), which is commonly required in food safety control,
biomedical analysis, public security, and many other disciplines.
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Keywords
bulk sample volumepork tissue samplesGCSingle sample analysisDirect Internal Extractive Electrospray Ionization-Mass Spectrometry Rapidfood safety controlaccuracy ratesβ- agonistsR 21 minQuantitative Determinationambient MSextractive electrospray ionization-mass spectrometryLODsample pretreatmentLCPork Tissue SamplesRSDBulk Molecular Concentrations