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Quantification of Proteins by Functionalized Gold Nanoparticles Using Click Chemistry

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journal contribution
posted on 20.02.2016, 21:47 by Kui Zhu, Yi Zhang, Sha He, Wenwen Chen, Jianzhong Shen, Zhuo Wang, Xingyu Jiang
This letter presents a click-chemistry-based assay for proteins (CAP) that allows quantitative determination of the concentration of proteins, using azide- and alkyne-functionalized gold nanoparticles (AuNPs). Compared with conventional methods, CAP has a broader linear range for detection of proteins with good selectivity. CAP enables the analysis of total proteins in various sera and milk samples.

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