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Pulmonary Insulin-like Growth Factor I Delivery from Trehalose and Silk-Fibroin Microparticles
journal contribution
posted on 2015-02-09, 00:00 authored by Isabel Schultz, Frederic Vollmers, Tessa Lühmann, Jens-Christoph Rybak, Ronja Wittmann, Katharina Stank, Hartwig Steckel, Boris Kardziev, Michael Schmidt, Petra Högger, Lorenz MeinelInsulin-like
growth factor I (IGF-I) is a strong anabolic peptide
with promising therapeutic value in muscle wasting diseases such as
sarcopenia. We report a pulmonary IGF-I delivery system deploying
silk-fibroin (SF) as carrier and in comparison to trehalose. Both
IGF-I delivery systems were characterized regarding IGF-I integrity,
IGF-I release profiles and aerodynamic properties. Transepithelial
in vitro transport of IGF-I using the pulmonary Calu-3 model cell
system followed comparable kinetics and mechanism of uptake as earlier
demonstrated for insulin (INS), for which effective pulmonary delivery
is known. Microparticles were spray-dried using either trehalose or
SF, resulting in geometries allowing alveolar deposition. The effective
IGF-I shuttling through the epithelial barrier of the lung was demonstrated
in an ex vivo human lung lobe model, and expanded the exciting possibility
of this administration route to this effective and anabolic peptide.