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Protein Recognition of Gold-Based Drugs: 3D Structure of the Complex Formed When Lysozyme Reacts with Aubipyc

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journal contribution
posted on 09.10.2014, 00:00 by Luigi Messori, Maria Agostina Cinellu, Antonello Merlino
The structure of the adduct formed in the reaction between Aubipyc, a cytotoxic organogold­(III) compound, and the model protein hen egg white lysozyme (HEWL) has been solved by X-ray crystallography. It emerges that Aubipyc, after interaction with HEWL, undergoes reduction of the gold­(III) center followed by detaching of the cyclometalated ligand; the resulting naked gold­(I) ion is found bound to the protein at Gln121. A direct comparison between the present structure and those previously solved for the lysozyme adducts with other gold­(III) compounds demonstrates that coordinated ligands play a key role in the protein–metallodrug recognition process. Structural data support the view that gold­(III)-based antitumor prodrugs are activated through metal reduction.