Probing Supramolecular Structure from Measurement of Methyl 1H−13C Residual Dipolar Couplings
journal contributionposted on 24.10.2007, 00:00 by Remco Sprangers, Lewis E. Kay
A simple NMR experiment is presented for the measurement of methyl 1H−13C residual dipolar couplings in fractionally aligned supramolecular protein complexes. The approach makes use of methyl-TROSY spectroscopy that significantly increases the lifetime of the NMR signal, along with highly deuterated, methyl-protonated samples that minimize relaxation contributions from protons external to the methyl group probes. An application to a 360 kDa half-proteasome complex is presented, establishing the methodology.