Pretreatment with γ‑Valerolactone/[Mmim]DMP and Enzymatic Hydrolysis on Corncob and Its Application in Immobilized Butyric Acid Fermentation
journal contributionposted on 08.10.2018, 00:00 by Wenxiu Zheng, Xujie Liu, Liying Zhu, He Huang, Tianfu Wang, Ling Jiang
Corncob is a widely available raw material with high carbohydrate and low lignin content. To improve corncob conversion to the fermentable sugars, a novel method encompassing pretreatment using the γ-valerolactone (GVL)/1-methyl-3-methylimidazolium dimethylphosphite ([Mmim]DMP) system integrated with cellulase hydrolysis was developed and optimized. It is confirmed that lignin was extracted efficiently after combined pretreatment and that the subsequent enzymatic saccharification efficiency could be significantly enhanced, resulting in the yield of 94.9% glucose from cellulose and 53.3% xylose from xylan, respectively. Furthermore, the above fermentable sugars were used as carbon source for Clostridium tyrobutyricum immobilized in macroporous Ca-alginate-lignin beads with the extracted lignin as the active ingredient to evaluate the fermentability of butyric acid. The results showed that high butyrate productivity of 0.47 g/L/h and yield of 0.45 g/g were obtained after 10 repeated batches of fermentation, demonstrating an effective process for the production of butyric acid from abundant corncob waste-biomass.