ol8b00121_si_001.pdf (1.2 MB)
Premutilin Synthase: Ring Rearrangement by a Class II Diterpene Cyclase
journal contribution
posted on 2018-02-01, 14:04 authored by Meimei Xu, Meirong Jia, Young J. Hong, Xihou Yin, Dean J. Tantillo, Philip J. Proteau, Reuben J. PetersBiosynthesis of the complex diterpenoid
antibiotic pleuromutilin
relies on a bifunctional (di)terpene synthase, and here site-directed
mutagenesis was used to knockout either of the two active sites. This
enabled characterization of the novel ring contracted intermediate
produced by the initiating class II diterpene cyclase active site.
Quantum chemical calculations further indicate the importance of reactant
configuration for this intriguing ring rearrangement.