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Potential of Ni(II)-NTA-Modified Poly(ethylene imine) Glycopolymers as Carrier System for Future Dendritic Cell-Based Immunotherapy

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journal contribution
posted on 10.03.2014, 00:00 by N. Hauptmann, M. Pion, R. Wehner, M.-Á. Muñoz-Fernández, M. Schmitz, B. Voit, D. Appelhans
Dendritic cells (DCs) play a crucial role in the development of cell-mediated immunotherapy due to their ability to induce and maintain strong immune responses. In our study, we evaluated a biocompatible Ni­(II)-NTA-modified poly­(ethylene imine) dendritic glycopolymer (Ni­(II)-NTA-DG) as new carrier system to increase the antigen uptake into iDCs for future DC-based immunotherapy. Ni­(II)-NTA-DG led to an increase in His6-Gp160 uptake in monocytes and iDCs, where His6-Gp160 is localized in the early endosomal and lysosomal compartments. Ni­(II)-NTA-DG and the formed polyplexes induced an activation of iDCs, showing an increasing expression of costimulatory molecules CD86, CD80, and proinflammatory cytokines IL-6 and IL-8. Beside no influencing effect of Ni­(II)-NTA-DG and polyplexes on the maturation of antigen-bearing DCs, the mature peptide bearing DCs remained their ability to migrate along a gradient of CCR7 ligands. Thus, Ni­(II)-NTA-DG with advancing biological properties is a promising carrier system for the future application in DC-based immunotherapy.