posted on 2019-06-20, 15:04authored byXueyuan Han, Linchun Mao, Wenjing Lu, Xiaopeng Wei, Tiejin Ying, Zisheng Luo
Wound-induced suberization
is an essentially protective healing
process for wounded fruit to reduce water loss and microbial infection.
It has been demonstrated that abscisic acid (ABA) could promote wound
suberization, but the molecular mechanism of ABA regulation remains
little known. In this study, the transcript level of Achn030011 (designated as AchnKCS), coding a β-ketoacyl-coenzyme
A synthase (KCS) involved in suberin biosynthesis, was found to be
significantly upregulated by ABA in wounded kiwifruit. A bZIP transcription
factor (Achn270881), a possible downstream transcription factor in
the ABA signaling pathway, was screened and designated as AchnbZIP12
according to its homology with related Arabidopsis transcription factors. A yeast one-hybrid assay demonstrated that
AchnbZIP12 could interact with the AchnKCS promoter.
Furthermore, significant trans-activation of AchnbZIP12 on AchnKCS was verified. The transcript level of AchnbZIP12 was also upregulated upon treatment with ABA. These results imply
that AchnbZIP12 acts as a positive regulator in ABA-mediated AchnKCS transcription during wound suberization of kiwifruit.