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Polysaccharide-Coated Thermosets for Orthopedic Applications: From Material Characterization to In Vivo Tests
journal contribution
posted on 2012-05-14, 00:00 authored by Andrea Travan, Eleonora Marsich, Ivan Donati, Marie-Pierre Foulc, Niko Moritz, Hannu
T. Aro, Sergio PaolettiThe long-term stability and success of orthopedic implants
depend
on the osseointegration process, which is strongly influenced by the
biomaterial surface. A promising
approach to enhance implant integration involves the modification
of the surface of the implant by means of polymers that mimic the
natural components of the extracellular matrix, for example, polysaccharides.
In this study, methacrylate thermosets (bisphenol A glycidylmethacrylate/triethyleneglycol
dimethacrylate), a widely used composition for orthopedic and dental
applications, have been coated by electrostatic deposition of a bioactive
chitosan-derivative. This polysaccharide was shown to induce osteoblasts
aggregation in vitro, to stimulate cell proliferation and to enhance
alkaline phosphatase activity. The coating deposition was studied
by analyzing the effect of pH and ionic strength on the grafting of
the polysaccharide. Contact angle studies show that the functionalized
material displays a higher hydrophilic character owing to the increase
of surface polar groups. The mechanical properties of the coating
were evaluated by nanoindentation studies which point to higher values
of indentation hardness and modulus (E) of the polysaccharide
surface layer, while the influence of cyclic stress on the construct
was assessed by fatigue tests. Finally, in vivo tests in minipigs
showed that the polysaccharide-based implant showed a good biocompatibility
and an ability for osseointegration at least similar to that of the
titanium Ti6Al4V alloy with roughened surface.
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Keywords
coating depositionbiomaterial surfacenanoindentation studiescyclic stressphosphatase activityfunctionalized material displaysOrthopedic Applicationsosseointegration processfatigue testsMaterial Characterizationcell proliferationindentation hardnesspolysaccharide surface layerosteoblasts aggregationimplant integrationvivo teststitanium Ti 6Al alloyextracellular matrixmethacrylate thermosetsContact angle studies showVivo TestsThe