Polyethyleneimine
Functionalized Mesoporous Magnetic
Nanoparticles with Enhanced Antibacterial and Antibiofilm Activity
in an Alternating Magnetic Field
posted on 2022-04-14, 16:03authored byWenhui Liu, Wenli Pei, Masoumeh Moradi, Dong Zhao, Zhong Li, Mingxing Zhang, Dake Xu, Fuhui Wang
Despite
a lot of research on the antibacterial effect of Fe3O4 nanoparticles, their interactions with biofilm
matrix have not been well understood. The surface charge of nanoparticles
mainly determines their ability to adhere on the biofilm. In this
work, negatively charged Fe3O4 nanoparticles
were synthesized via a trisodium citrate-assisted solvothermal method
and then the surfaces were functionalized using polyethyleneimine
(PEI) to obtain positively charged Fe3O4 nanoparticles.
The antibacterial and antibiofilm activities of both negatively and
positively charged Fe3O4 nanoparticles in an
alternating magnetic field were then systematically investigated.
The positively charged Fe3O4 nanoparticles showed
a strong self-adsorbed attachment ability to the planktonic and sessile
cells, resulting in a better antibacterial activity and enhanced biofilm
eradication performance compared to the conventional Fe3O4 nanoparticles with negative charges. Fe3O4@PEI nanoparticles produced physical stress and thermal
damage in response to an alternating magnetic field, inducing the
accumulation of intracellular reactive oxygen species into live bacterial
cells, bacterial membrane damage, and biofilm dispersion. Utilizing
an alternating magnetic field along with positively charged nanoparticles
leads to a synergistic antibacterial approach to improve the antibiofilm
performance of magnetic nanoparticles.