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Polyamine-Modified Gold Nanoparticles Readily Adsorb on Cell Membranes for Bioimaging
journal contribution
posted on 2019-10-17, 15:55 authored by Xiang-Fu Du, Bing-Jie Zhu, Zhong-Chao Cai, Chaojie Wang, Mei-Xia ZhaoThe surface modification
of nanoparticles (NPs) can enhance cellular
and intracellular targeting. A new type of polyamine-modified gold
NPs (AuNPs) are designed and synthesized, which can be selectively
absorbed onto the cell membrane. AuNPs with an average diameter of
4.0 nm were prepared and modified with polyamine (R-4C) through amidation.
In order to detect the distribution of NPs within cells by fluorescence
imaging, AuNP@MPA-R-4C was functionalized with fluorescein isothiocyanate
(FITC). The fluorescence-labled NPs AuNP@MPA-R-4C-FITC demonstrated
minimal cytotoxicity in several cell lines. Both confocal laser scanning
microscopy and transmission electron microscopy demonstrated that
AuNP@MPA-R-4C-FITC was distributed on the cell membrane. Compared
with the free organic dye, the modified AuNPs showed significantly
increased accumulation on the cell membrane after treatment for only
10 min. These results suggested that AuNP@MPA-R-4C-FITC can be used
as a bioprobe targeting the cell membrane for various biological applications.