ol9b01526_si_001.pdf (1.42 MB)
Download filePolyacetate and Polycarbonate RNA: Acylating Reagents and Properties
journal contribution
posted on 2019-07-03, 00:00 authored by Maryam Habibian, Willem A. Velema, Anna M. Kietrys, Yoshiyuki Onishi, Eric T. KoolAcylation
of RNA at 2′-OH groups is widely applied in mapping RNA structure
and recently for controlling RNA function. Reactions are described
that install the smallest 2-carbon acyl groups on RNAnamely,
2′-O-acetyl and 2′-O-carbonate groups. Hybridization
and thermal melting experiments are performed to assess the effects
of the acyl groups on duplex formation. Both reagents can be employed
at lower concentrations to map RNA secondary structure by reverse
transcriptase primer extension (SHAPE) methods.