posted on 2015-01-12, 00:00authored byCamille Legros, Anne-Laure Wirotius, Marie-Claire De Pauw-Gillet, Kam Chiu Tam, Daniel Taton, Sébastien Lecommandoux
Hydrophilic nanogels based on partially
hydrolyzed poly(2-ethyl-2-oxazoline)
were synthesized in dilute aqueous media in the presence of 1,6-hexanediol
diglycidyl ether as a cross-linker. Nanogel formation was monitored
by DLS and HSQC NMR spectroscopy, and the final nano-objects were
characterized by DLS, TEM, AFM, and NanoSight analyses. Nanogels with
a hydrodynamic radius of 78 nm exhibiting a slight positive surface
charge were obtained. MTS assays (cell metabolic activity test) evidenced
that nanogels were nontoxic in the investigated concentration range
(i.e., 0.1 to 400 μg/mL) and that no specific interaction with
bovine serum albumin was observed.